Syslinux can be run under many different Windows operating environments including DOS. The following document lists a couple examples of how to use syslinux depending on your operating environment. Note that these paths may change with newer releases of syslinux and this information is being provided for reference only.
How to Run Syslinux from Windows 98 or ME
Quoted per the Syslinux Documentation;
Use “syslinux.com” (in the dos subdirectory of the distribution) for
plain DOS (MS-DOS, DR-DOS, PC-DOS, FreeDOS…) or Win9x/ME.
Use “syslinux.exe” (in the win32 subdirectory of the distribution) for